Z-Pro-Prolinal
Also known as: Cbz-Pro-Prolinal, N-benzyloxycarbonyl-L-prolyl-L-prolinal, Z-Pro-Prolinal, ZPP
Summary
Z-Pro-Prolinal (ZPP) is a synthetic dipeptide aldehyde used extensively as a selective, potent research tool inhibitor of prolyl endopeptidase. It has been employed in preclinical studies to probe the role of PEP in neuropeptide metabolism, memory, cognition, and psychiatric conditions. It is not approved for clinical use.
Mechanism of Action
Competitive inhibitor of prolyl endopeptidase (PEP, EC 3.4.21.26), a serine protease that cleaves peptide bonds on the carboxyl side of proline residues. Z-Pro-Prolinal forms a reversible covalent hemiacetal with the active-site serine, blocking hydrolysis of proline-containing neuropeptides such as substance P, neurotensin, and thyrotropin-releasing hormone.
Routes of Administration
Goals & Uses
- Neuropeptide metabolism researchNeuroscience / BiochemistryModerate
- Psychiatric disorder modelingPreclinical Disease ModelingLow
- Prolyl endopeptidase inhibitionEnzyme Inhibition / Pharmacological ToolHigh
- Cognitive and memory researchNeuropharmacologyModerate
Contraindications
- Human clinical useRegulatoryHigh
Adverse Effects
- Off-target protease inhibitionPharmacologicalUnknown
- Behavioral alterationsNeurological (preclinical)Unknown
Drug Interactions
No drug interactions recorded yet.
Population Constraints
- All human populationsGeneralAbsolute
Regulatory Status
- European UnionUnapprovedResearch chemical only; no CTA filed.
- United StatesUnapprovedResearch chemical only; no IND filed.
- United KingdomUnapprovedResearch chemical only; no clinical authorisation.
No regulatory approval in any jurisdiction. Used exclusively as a biochemical research tool. Not an approved drug or investigational medicinal product under IND/CTA.
Evidence & Sources
No sources recorded yet.