S-(2,4-dinitrophenyl)glutathione

Summary

S-(2,4-dinitrophenyl)glutathione is a synthetic glutathione conjugate produced enzymatically from glutathione and 1-chloro-2,4-dinitrobenzene via glutathione S-transferase catalysis. It serves primarily as a biochemical research tool and probe for measuring GST activity in cell lysates and for studying MRP1/MRP2-mediated export mechanisms. It has no clinical application.

Mechanism of Action

S-(2,4-dinitrophenyl)glutathione (DNP-SG) is formed via conjugation of glutathione with 1-chloro-2,4-dinitrobenzene (CDNB) catalyzed by glutathione S-transferases (GSTs). It acts as a substrate and inhibitor of multidrug resistance-associated proteins (MRPs/ABCCs), particularly MRP1 and MRP2, which actively transport it out of cells. It is used as a probe to study GST activity, MRP-mediated transport, and cellular detoxification pathways.

Routes of Administration

No administration routes recorded yet.

Goals & Uses

• Multidrug resistance investigationResearch / OncologyModerate
• GST activity measurementResearch / Biochemical AssayHigh
• Cellular detoxification pathway studiesResearch / ToxicologyModerate
• MRP1/MRP2 transport characterizationResearch / Transporter BiologyHigh

Contraindications

• Human therapeutic useRegulatory/safetyHigh

Adverse Effects

• Cytotoxicity (in vitro)ToxicityCommon

Drug Interactions

• MRP1/ABCC1 inhibitors (e.g., MK-571)Low

Population Constraints

• All human populationsGeneralAbsolute

Regulatory Status

• European UnionUnapprovedNo EMA regulatory status; used solely as a laboratory biochemical.
• United StatesUnapprovedResearch reagent only; not reviewed or approved by the FDA for any therapeutic indication.
• United KingdomUnapprovedNo MHRA regulatory status; research use only.

No regulatory approval in any jurisdiction. Used exclusively as a biochemical research reagent. Not intended for human or veterinary therapeutic use.

Evidence & Sources

No sources recorded yet.